A Reducing Substance in Human Urine Resembling Glucose

Partial separation of gonadotropin-inhibiting substance in human urine.

The chromatographic separation of gonadotropin-inhibiting substance in the extract obtained from adult male urine according to the method of Johnsen was attempted on a Sephadex G-100 column. The ovulation assay was employed to detect the inhibitor in the fractions using female mice, 19-20 days old, which received PMS and HCG to obtain a minimum constant ovulation. In the chromatography of the e...

The estimation of a fraction resembling pregnanediol glucuronide in human urine.

The amount of pregnanediol in human urine can be estimated by an assay of the (partially purified) sodium pregnanediol glucuronidate (NaPG) extracted by butanol (Venning, 1937, 1938), or of the pregnanediol itself, extracted from the urine after hydrolytic treatment (Astwood & Jones, 1941; Talbot, Berman, MacLachlan & Wolfe, 1941). The latter method is capable of considerable specificity and se...

Stability of glucose in urine.

CLINICAL CHEMISTRY, Vol. 26, No. 6, 1980 789 interference with analysis for theophylline by high-performance liquid chromatography. Clin. Chem. 24, 1847 (1978). Letter. 4. Kelly, R. C. Prentice, D. E., and Hearne, G. M., Cephalosporin antibiotics interfere with the analysis for theophylline by highpressure liquid chromatography. Clin. Chem. 24, 838 (1978). Letter. 5. Orcutt, J. S., Kozak, P. P....

A Comparison of Pituitrin with the Antidiuretic Substance Found in Human Urine and Placenta.

ENU mutagenesis identifies mice with mitochondrial branched-chain aminotransferase deficiency resembling human maple syrup urine disease.

Tandem mass spectrometry was applied to detect derangements in the pathways of amino acid and fatty acid metabolism in N-ethyl-N-nitrosourea-treated (ENU-treated) mice. We identified mice with marked elevation of blood branched-chain amino acids (BCAAs), ketoaciduria, and clinical features resembling human maple syrup urine disease (MSUD), a severe genetic metabolic disorder caused by the defic...